Dear Mohamed,
You should be able to measure the delay between input and output pulses in the fiber. You may try dual-port scope in the electrical or optical domain depending on your application.
There is also Photonics All-parameter Analyzer that can be used for such measurements.
Backscattered signal is not a localized signal. It is only a time domain random signal representing the Rayleigh scattering. This signal can’t be used for OTDR application. However, OptiSystem has an OTDR component that allows users to design the system.